Nurturing Life Sciences
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#Unit 6. System Physiology – Plant
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#Unit 10. Ecological Principles
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#Unit 13. Methods in Biology
Touchdown PCR is initiated at very high annealing temperatures, which allow only perfectly matched primer-template DNA hybrids to form and support amplification. The annealing temperature is dropped in a stepwise fashion with each cycle of PCR.
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#Unit 3. Fundamental Processes
Humans express four Argonaute proteins. AGO2 is the human Argonaute protein in miRNA-containing RISC complexes.
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The PCR primers must not have self complementary regions as this would lead to hairpin formation within primer molecules and make then unsuitable for PCR.