Arrestin binds tightly to rhodopsin only when rhodopsin kinase has phosphorylated a sufficient number of C-terminal serines and threonines.

TLS Online TPP Program

#Id: 871

#Unit 13. Methods in Biology

Variants of PCR has been devised to enhance efficiency are 

a. qPCR with SYBR, Taqman and MB 

b. Nested PCR

c. Touchdown PCR

d. Hot-start PCR 

TLS Online TPP Program

#Id: 872

#Unit 13. Methods in Biology

Variants of PCR has been devised for sequence specific amplification are Inverse PCR, Anchored PCR, RT-PCR RACE (3’ and 5’) RLM-RACE, Multiplex PCR, Colony PCR.

TLS Online TPP Program

#Id: 873

#Unit 13. Methods in Biology

Variants of PCR has been devised for mutational analysis AS-PCR, Site-directed-mutagenesis (Oligonucleotide-directed Mutagenesis), overlap extension PCR, Megaprimer PCR.

TLS Online TPP Program

#Id: 874

#Unit 13. Methods in Biology

Touchdown PCR, has been devised to increase the specificity of PCR without lowering the efficiency by variation annealing temperature.

TLS Online TPP Program

#Id: 875

#Unit 13. Methods in Biology

HOT START PCR were devised to prevent non – specific amplification at low temperature.

TLS Online TPP Program

#Id: 876

#Unit 13. Methods in Biology

FRET is possible only when the distance between donor and acceptor are within 1 to 10 Å