The PCR technique widely used in bacterial genomic studies for screening of plasmid inserts is

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#Question id: 24437

#Unit 1. Molecules and their Interaction Relevant to Biology

If bovine mitochondria have 8 c subunits per c ring, what is the predicted ratio of ATP formed per NADH oxidized?

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#Question id: 11477

#Unit 7. System Physiology – Animal

How could you genetically modify an animal so that it would distinguish more shades of green?

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#Question id: 16126

#Unit 13. Methods in Biology

2 You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS, that could explain the effect of the gln2– and gln3– mutations on the regulation of GLN1.

The GLN1 gene shows a rather complex regulation in response to different amino acids. When either glutamate (glu) or glutamine (gln) is added to the medium, the amount of GS expression diminished and when both glutamate and glutamine are added to the medium GS expression is shut off completely. The effects of different mutants on the response to glu and gln are shown below.

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#Question id: 4542

#Unit 3. Fundamental Processes

Repression of the GAL1 gene in yeast by Mig1, in the presence of glucose follows those steps;

I- Deacetylation of local nucleosomes

II-Recruiting the Tup1 repressing complex

III- Mig1 represses expression of GAL1

IV- Mig1 binds a site between the UASG and the GAL1 promoter

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#Question id: 12834

#Unit 2. Cellular Organization

How Aurora B and the spindle assembly checkpoint pathway collaborate during every cell cycle to accurately attach every single pair of sister chromatids to the mitotic spindle in the correct, bioriented manner?