TLS Online TPP Program

#Question id: 18853


Your purification strategy of combinations of chromatography steps gives a protein preparation with a single band on SDS-PAGE. Which of the following would be best for determining the protein concentration (as mg/ml or molarity)?

#Unit 14. Methods in Biology
  1. Measure a UV absorbance scan and use the absorbance at 280nm with the molar extinction coefficient (predicted from the amino acid sequence)
  2. Determine amino acid composition after hydrolysis to amino acids
  3. Colorimetric assay using Bradford or BCA assays
  4. "Guesstimate" the concentration from the intensity of bands on SDS-PAGE gel
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TLS Online TPP Program

#Question id: 20260

#Unit 13. Advances in Biotechnology

The modified forms of starch have varied applications in food and other industries. Basic strategies to modify starch quality are 
(i) genetic modification of crops (by breeding or biotechnology)
(ii) food processing (using enzymes and chemicals)
(iii) food supplementation (modification of starch by employing microorganisms)
Which of the following strategies are correct?

TLS Online TPP Program

#Question id: 20261

#Unit 13. Advances in Biotechnology

Certain modified forms of starch such as high amylose starch are widely used in 
1. Snack products
2. Improve uniformity, stability and texture
3. Thickener
Which of the following are correct?

TLS Online TPP Program

#Question id: 20262

#Unit 13. Advances in Biotechnology

Certain modified forms of starch such as high amylopectin starch are widely used in 
1. Snack products
2. Improve uniformity, stability and texture
3. Thickener
4. Paper industry
Which of the following is correct option?

TLS Online TPP Program

#Question id: 20263

#Unit 13. Advances in Biotechnology

_______  an excellent alternative to polystyrene-based packing material

TLS Online TPP Program

#Question id: 20264

#Unit 13. Advances in Biotechnology

______________ is the main enzyme of amylose biosynthesis. Whose increased activity is expected to increase the amylose content of starch?

TLS Online TPP Program

#Question id: 20336

#Unit 13. Advances in Biotechnology

Granule bound starch synthase I (GBSS l) is the main enzyme of amylose biosynthesis. Therefore ___________ activity of GBSS I is expected to increase the amylose content of starch