TLS Online TPP Program

#Question id: 18853


Your purification strategy of combinations of chromatography steps gives a protein preparation with a single band on SDS-PAGE. Which of the following would be best for determining the protein concentration (as mg/ml or molarity)?

#Unit 13. Methods in Biology
  1. Measure a UV absorbance scan and use the absorbance at 280nm with the molar extinction coefficient (predicted from the amino acid sequence)
  2. Determine amino acid composition after hydrolysis to amino acids
  3. Colorimetric assay using Bradford or BCA assays
  4. "Guesstimate" the concentration from the intensity of bands on SDS-PAGE gel
More Questions
TLS Online TPP Program

#Question id: 7286

#Unit 5. Developmental Biology

Which of the floral whorls is affected in apetala 3/pistillata (ap3/pi) mutants?

TLS Online TPP Program

#Question id: 7287

#Unit 5. Developmental Biology

Following statements are regarding to the the floral quartet model and the underlying ABCDE model of organ identity determination in Arabidopsis thaliana.

A. Five floral organ identities (sepals, petals, stamens, carpels, and ovules) are specified by the formation of floral organ-specific tetrameric complexes of MADS-domain transcription factors that bind to two nearby enhancer elements, forming a DNA loop in between.

B. A complex of two class A proteins (APETALA 1) and two class E proteins  (SEPALLATA) determines petal identity.

C. A complex of one class A protein, one class E protein, and one of each of the class B proteins (APETALA 3 and PISTILLATA) determines sepal identity.

D. A complex of one class E protein, two class B proteins, and one class C protein (AGAMOUS) determines stamen identity, while a complex of two class E proteins and two class C proteins determines carpel identity.D. A complex of one class E protein, two class B proteins, and one class C protein (AGAMOUS) determines stamen identity, while a complex of two class E proteins and two class C proteins determines carpel identity.

E. A complex of one class E protein, one class C protein, and one of each of the class D proteins (SHATTERPROOF and SEEDSTICK) controls ovule identity.

Which of the following combination are correct?

TLS Online TPP Program

#Question id: 7288

#Unit 5. Developmental Biology

Following statements are regarding to the expression of genes in floral quartate model in Arabidopsis thaliana.

A. Sepals- AP1(heterodimer) + SEP(homodimer)

B. Petals-AP1(monomer)+PI(monomer)+AP3(monomer)+SEP(monomer)

C. Stamens- AP3(monomer)+PI(monomer)+AG(monomer)+SEP(monomer)

D. Carpels- AG(homodimer)+SEP(homodimer)

Which of the following genetic combinations are incorrect?

TLS Online TPP Program

#Question id: 7289

#Unit 5. Developmental Biology

The following scheme shows the flowering status of a plant species and the photoperiodism. Which of the following conclusions is most appropriate?

TLS Online TPP Program

#Question id: 7289

#Unit 1. Molecules and their Interaction Relevant to Biology

The following scheme shows the flowering status of a plant species and the photoperiodism. Which of the following conclusions is most appropriate?

TLS Online TPP Program

#Question id: 7290

#Unit 5. Developmental Biology

Following figure regarding to regulatory network in the Arabidopsis shoot meristem stem-cell niche.

A. The transcription factor WUS is synthesized in the cell A and moves via plasmodesmata into cell B, where one of its functions is to induce expression of the CLV3.

B. The transcription factor CLV3 is synthesized in the cell A and moves via plasmodesmata into cell B, where one of its functions is to induce expression of the WUS.

C. Secreted CLV3 protein from cell B binds to the receptor protein kinase on the surface of cell A; there, it activates a signal that represses WUS transcription.

D. Secreted WUS protein from cell B binds to the receptor protein kinase on the surface of cell A; there, it activates a signal that represses CLV3 transcription.

Which of the following combination is correct?