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#Question id: 18853


Your purification strategy of combinations of chromatography steps gives a protein preparation with a single band on SDS-PAGE. Which of the following would be best for determining the protein concentration (as mg/ml or molarity)?

#Unit 13. Methods in Biology
  1. Measure a UV absorbance scan and use the absorbance at 280nm with the molar extinction coefficient (predicted from the amino acid sequence)
  2. Determine amino acid composition after hydrolysis to amino acids
  3. Colorimetric assay using Bradford or BCA assays
  4. "Guesstimate" the concentration from the intensity of bands on SDS-PAGE gel