The enzyme aldolase, catalyzes a reversible aldol condensation, Fructose 1,6-bisphosphate is cleaved to yield two different triose phosphates, glyceraldehyde 3-phosphate, an aldose, and dihydroxyacetone phosphate, a ketose. There are two classes of aldolases such as;

A) Class I aldolases

B) Class II aldolases

i) Found in animals and plants

ii) Found in in fungi and bacteria

iii) formation of protonated Schiff base on enzyme and its active site contain lysine residue

iv) do not form the Schiff base intermediate and a zinc ion at the active site is coordinated with the carbonyl oxygen at C-2

In which of the following option is the given enzyme with its correct statements?

TLS Online TPP Program

#Question id: 18846

#Unit 13. Methods in Biology

For an application where you require a sample of your target protein at high purity, what would be a good purification strategy? Assume that your starting point is E. coli cells in which the target protein fused to an affinity tag has been over-expressed.

TLS Online TPP Program

#Question id: 18847

#Unit 13. Methods in Biology

You know that the protein you want to purify from a natural source forms a multimer with multiple sub-units giving a molecular weight in solution much bigger than visualised denatured on SDS-PAGE. There is only a small amount of the target protein in the total protein sample. Which of the following is an appropriate purification strategy?

TLS Online TPP Program

#Question id: 18848

#Unit 13. Methods in Biology

You find that your protein sample loses activity during storage. What can you do about this?

TLS Online TPP Program

#Question id: 18849

#Unit 13. Methods in Biology

Which of these techniques is often considered a suitable "polishing" step in a protein purification strategy?

TLS Online TPP Program

#Question id: 18850

#Unit 13. Methods in Biology

Which of these chromatography types are suitable as a "capture" step in the purification of non-tagged proteins?

TLS Online TPP Program

#Question id: 18851

#Unit 13. Methods in Biology

Nickel-NTA (Ni-NTA) chromatography is a popular affinity chromatography method for the purification of histidine-tagged proteins. However, SDS-PAGE of the eluted protein can show bands in addition to your target protein. How might you improve this purification step?