TLS Online TPP Program

#Question id: 12718


primary disruptions could be signaling the plant that a change in environmental conditions has occurred and that it’s time to respond by altering existing pathways or by activating stress-response pathways. At least five different types of stress-sensing mechanisms can be distinguished:

                       

                          COLUMN I

 

 

                              COLUMN II

 

A) Physical sensing

 

 

i) usually results from the detection of by-products that accumulate in cells due to the uncoupling of enzymatic or electron transfer reactions, such as the accumulation of ROS during stress caused by too much light.

 

 

B) Biophysical sensing

 

 

ii) often involves the presence of specialized proteins that have evolved to sense a particular stress; for example, calcium channels that can sense changes in temperature and alter Ca2+ homeostasis.

 

 

C) Metabolic sensing

 

 

iii) refers to modifications of DNA or RNA structure that do not alter genetic sequences, such as the changes in chromatin that occur during temperature stress.

 

 

D) Biochemical sensing

 

 

iv) refers to the mechanical effects of stress on the plant or cell structure, for example, the contraction of the plasma membrane from the cell wall during drought stress.

 

 

E) Epigenetic sensing

 

 

v) might involve changes in protein structure or enzymatic activity, such as the inhibition of different enzymes during heat stress.

 

 

  
Match the correct sequence sensing mechanism during in stress;

#Unit 6. System Physiology – Plant
  1. A-iii, B-i, C-iv, D-v, E-ii
  2. A-v, B-iii, C-ii, D-i, E-iv
  3. A-ii, B-iv, C-v, D-iii, E-i
  4. A-iv, B-v, C-i, D-ii, E-iii
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TLS Online TPP Program

#Question id: 5290

#Unit 8. Inheritance Biology

The following table shows mapping data from three interrupted mating experiments using three different Hfr strains and an F- strain: The order of genes is

TLS Online TPP Program

#Question id: 5291

#Unit 8. Inheritance Biology

A number of nutritional mutant strains were isolated from wild-type Neurospora that responded to the addition of certain supplements in the culture medium by growth ( + ) or lack of growth ( -). Given the following responses for single gene mutations, diagram a metabolic pathway that could exist in the wild-type strain that is most consistent with the data. Indicate where the pathway is blocked in each mutant strain.

TLS Online TPP Program

#Question id: 5292

#Unit 8. Inheritance Biology

A transformation experiment is performed with a donor strain that is resistant to four drugs: W, X, Y, and Z. The recipient is sensitive to all four drugs. The treated recipient population is divided up and plated on media containing various combinations of the drugs. The table below shows the results:

Drug(s) added

Number of Colonies

None

20,000

WX

76

WY

1324

WZ

1976

XY

85

XZ

69

YZ

1564

WXY

50

WXZ

62

WYZ

1354

XYZ

48

WXYZ

53

Following observation are given

A) X gene is obviously quite distant from the other three genes

B) Y gene is obviously quite distant from the other three genes

C) The Order is three closely link gene W Z Y

D) The Order is three closely link gene W X Z

Which of the following combination is correct?

TLS Online TPP Program

#Question id: 5293

#Unit 8. Inheritance Biology

The two strains a and b are tested for being auxotrophic for the following nutritional mutations: pyridoxine (pdx), biotin (bio), methionine (met), adenine (ade), and para-aminobenzoic acid (pab). Growth is indicated by ( + ) and lack of growth by ( - ). From these tests, what is genotype of strain a and b respectively (+ indicate prototroph while other auxotroph)? 

TLS Online TPP Program

#Question id: 5294

#Unit 8. Inheritance Biology

Interrupted mating experiments were performed using three different Hfr strains (1-3). The three strains have different combinations of selectable markers. The time of entry for markers for each strain is shown is shown in the table given.

Hfr 1

Phe

(5)

Trp

(35)

Met

(50)

Ala

(52)

Hfr 2

Trp

(2)

Val

(12)

His

(57)

Pro

(87)

Hfr 3

Ala

(10)

Met

(12)

Val

(37)

Phe

(57)

Using the above data, predict the correct sequence of markers on the E. coli chromosome

TLS Online TPP Program

#Question id: 5295

#Unit 8. Inheritance Biology

A cross is made between an Hfr that is met+ thi+ pur+ and an F-that is met- thi- pur-. Interrupted mating studies show that met+ enters the recipient last, so met+ recombinants are selected on a medium containing supplements that satisfy only the pur and thi requirements.  These recombinants are tested for the presence of the thi+ and pur+ alleles. The following numbers of individuals are found for each genotype:

A) Gene Order

I

Thi Pur  Met

II

Pur  Met  Thi

III

Pur   Thi  Met

B) Distance between Pur and Thi

IV

15.4 map unit

V

1.8 map unit

What is the following correct combination?