A cylindrical stirred bioreactor of diameter and height 2 m has a Rushton turbine one-third the tank diameter in size. The bioreactor contains Newtonian culture broth with the same density as water and with viscosity 4 cP. If the specific power consumption must not exceed 1.5 kW m^-3, determine  the maximum allowable stirrer speed. What is the mixing time under these conditions?  __________

#Question id: 533

#Section 4: Fundamentals of Biological Engineering

Glucose, labeled with 14C in different carbon atoms, is added to a crude extract of a tissue rich in the enzymes of the pentose phosphate pathway.  The most rapid production of 14CO2 will occur when the glucose is labeled in:

#Question id: 535

#Section 4: Fundamentals of Biological Engineering

In some diabetic patients, glucose increases disproportionately and is unresponsive to an insulin challenge; under these conditions, how would oneʹs liver normally respond?

A) phosphorylating glucose for entry into the glycolytic pathway

B) saturating glucokinase with glucose

C) phosphorylating glucose for entry into the glycogen synthesis pathways

#Question id: 538

#Section 4: Fundamentals of Biological Engineering

Which of the following statements are correct about cells that form ATP mainly by glycolysis are

A) anaerobic yeasts.

B) lactic acid bacteria.

C) kidney medulla cells.

#Question id: 540

#Section 4: Fundamentals of Biological Engineering

During strenuous exercise, the NADH formed in the glyceraldehyde 3-phosphate dehydrogenase reaction in skeletal muscle must be reoxidized to NAD+ if glycolysis is to continue.  The most important reaction involved in the reoxidation of NADH is:

#Question id: 541

#Section 4: Fundamentals of Biological Engineering

The overall △G for glycolysis is -72 kJ/mol in erythrocytes. Which statement below is true?

A) The value of △G0ʹ is also -72 kJ/mol since the cytosol pH is close to 7.

B) The free energy of glycolysis is found as the sum of the standard free energy changes for

the individual pathway reactions.

C) The negative sign of △G shows that this pathway will proceed toward product (pyruvate) under normal cellular conditions.

#Question id: 542

#Section 4: Fundamentals of Biological Engineering

Hamsters love to run on exercise wheels. Prolonged running at a high rate of speed requires ATP. Could a hamster with a defective gene for the enzyme lactate dehydrogenase meet the extra ATP demand for prolonged, fast wheel-running by maintaining a high rate of glycolysis? Why or why not?