A cylindrical stirred bioreactor of diameter and height 2 m has a Rushton turbine one-third the tank diameter in size. The bioreactor contains Newtonian culture broth with the same density as water and with viscosity 4 cP. The tank is now aerated. In the presence of gas bubbles, the approximate relationship between ungassed power number (N_P)₀ and gassed power number (N_P)g is: (N_P)g =0.5 (N_P)₀. What maximum stirrer speed is now possible in the sparged reactor? Estimate the mixing time.

TLS Online TPP Program

#Question id: 12905

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Some gene/protein is used to identify the Disorder in humans Match them correctly:

1. Tyrosine Kinase(TK)	i. Atherosclerosis
2. HIV transactivator	ii. Hypertension
3. Angiotensinogen	iii. Kaposis Sarcoma
4. CET protein	iv. Cardiac Hypertrophy

TLS Online TPP Program

#Question id: 12906

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

An alternative method labeling strategy used in karyotyping and gene localisation is _______

TLS Online TPP Program

#Question id: 12907

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The binding of a regulatory protein or transcription factor to a specific DNA site results in a complex which may be analyzed by the technique termed as _____

TLS Online TPP Program

#Question id: 12908

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which statement is incorrect about CRISPR?

TLS Online TPP Program

#Question id: 12909

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

TLS Online TPP Program

#Question id: 12910

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:

A. RNA analysis	i. Sexing or cell mutation sites	a. Expressed Sequence tag
B. Forensic science	ii. mRNA analysis	b. Analysis of DNA from blood
C. Gene discovery	iii. DNA profiling	c. Active latent viral infections
D. Single-cell analysis	iv. RT–PCR	d. Sex determination of unborn