TLS Online TPP Program

#Question id: 4345


For a repressible operon to be transcribed, which of the following conditions must occur?

#Section 3: Genetics, Cellular and Molecular Biology
  1. A corepressor must be present.

  2. RNA polymerase and the active repressor must be present.

  3. RNA polymerase must bind to the promoter, and the repressor must be inactive.

  4. RNA polymerase must not occupy the promoter, and the repressor must be inactive.

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TLS Online TPP Program

#Question id: 27356

#Section 3: Genetics, Cellular and Molecular Biology

What is the role of the guide RNA?

TLS Online TPP Program

#Question id: 27359

#Section 5: Bioprocess Engineering and Process Biotechnology

A double reciprocal plot was created from the specific growth rate and limiting-substrate concentration data obtained from a chemostat experiment. A linear regression gave values of 1.25 h and 100 mg hL-1 for the intercept and slope, respectively.
(a) The respective values of the Monod kinetic constants μm (h-1) and K. (mg L-¹) are as follows:

TLS Online TPP Program

#Question id: 27360

#Section 5: Bioprocess Engineering and Process Biotechnology

The same culture (with the m and K, values as computed above) is cultivated in a 10 L chemostat being operated with a 50 mL min-1 sterile feed containing 50 g L-1 of substrate. Assuming an overall yield coefficient of 0.3 (g- DCW) (g- substrate), the respective values of the outlet biomass and substrate concentrations are

TLS Online TPP Program

#Question id: 27361

#Section 5: Bioprocess Engineering and Process Biotechnology

A continuous stirred tank bioreactor produces 48 kg lysine.day-1. If the volumetric productivity is 2 g.l-1.h-1, the volume of the reactor is:

TLS Online TPP Program

#Question id: 27362

#Section 5: Bioprocess Engineering and Process Biotechnology

Continuous stirred tank fermenter is characterized by (biomass concentration X kg/m3, residual substrate is S kg/m3, Sf=substrate concentration in feed kg/m3, Dilution rate = D and µm>>D and Km<Sf, XD= the biomass productivity kg/m3h. If the dilution rate is doubled to 2D then the new steady state values in comparison with earlier steady state is characterized by

TLS Online TPP Program

#Question id: 27363

#Section 3: Genetics, Cellular and Molecular Biology

Match the types of RNA in Group I with their corresponding function in Group II