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TLS Online TPP Program

#Question id: 13052

What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology
  1. PCR would proceed normally
  2. Non-specific PCR of random templates will occur
  3. Non-specific PCR of random templates will occur
  4. The PCR reaction will not commence
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TLS Online TPP Program

#Question id: 15866

#Section 6: Plant, Animal and Microbial Biotechnology

Tissues and cells cultured in a liquid medium produce a single cells and cells clumps of few to many cells; these are called;
TLS Online TPP Program

#Question id: 15867

#Section 6: Plant, Animal and Microbial Biotechnology

Which of the following culture is used for the production of primary and secondary metabolites?
TLS Online TPP Program

#Question id: 15868

#Section 6: Plant, Animal and Microbial Biotechnology

If the culture is kept in stationary phase for a prolonged period, the cells may die. Why?
a) due to the time taken for doubling of cell number
b) duration of a cell generation is too large, depending mainly on the plant species.
c) stationary phase is forced on the culture by a depletion of the nutrients
d) due to an accumulation of cellular wastes
TLS Online TPP Program

#Question id: 15869

#Section 6: Plant, Animal and Microbial Biotechnology

In a modest laboratory, aseptic handling, maintenance and observation of cultures, these activities can be done in the culture room.  Some facilities are required for the culture room, except?
TLS Online TPP Program

#Question id: 15870

#Section 6: Plant, Animal and Microbial Biotechnology

 What is the advantage of plant tissue culture over animal tissue culture?
TLS Online TPP Program

#Question id: 15871

#Section 6: Plant, Animal and Microbial Biotechnology

Various sterilization techniques used in plant tissue culture work;

           Sterilization technique

 

          Materials sterilized

     A) Dry heat

i) Media, culture vessels, other glass plasticwarc, contaminated cultures

 

      B) Autoclaving

ii) Heat labile compounds like GA3, ABA, zeatin, urea,·enzymes, etc.

 

      C) Surface sterilization

iii) Empty glassware, instruments like scalpels, forceps, needles

 

     D) Liquid (membrane filter of 

       0.45 μm or smaller pore size)

iv) All plant materials to be cultured