What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

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#Question id: 13035

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Each band on the gel represents a different protein (or protein subunit); _____ proteins move through the gel more _____ than_____ proteins and therefore are found nearer the _____ of the gel.

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#Question id: 13036

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

In isoelectric focusing the protein will move until

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#Question id: 13037

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following proteins will be most far away from the positively charged electrode during isoelectric focusing in a buffer with pH of 5?

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#Question id: 13038

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Please arrange the following steps about ELIZA (Enzyme-linked immunosorbent assay) in chronological order

i. incubate with antibody-enzyme complex that binds primary antibody

ii. coat surface with antigen, block unoccupied sites with nonspecific protein

iii. add substrate, formation of colored product indicates presence of specific antigen

iv. incubate with primary antibody against specific antigen

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#Question id: 13039

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

 Which of the following is incorrect about a microarray?

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#Question id: 13040

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which of the following is incorrect about Oligonucleotide Design in A microarray?