Choose the correct order for the steps involve in Insitu hybridization.

1. Cell permeaabilisation

2. Detection of hybridised probe radioactive.

3. Wash excess probe from section

4. Cell fixation

#Question id: 28113

#Section 5: Bioprocess Engineering and Process Biotechnology

#Question id: 28114

#Section 5: Bioprocess Engineering and Process Biotechnology

Which is the most frequently used impeller in the fermentation industry regarded as 6- flat-blade disc-mounted turbine?

#Question id: 28115

#Section 5: Bioprocess Engineering and Process Biotechnology

Transient flow range of mixing speed is obtained for

#Question id: 28116

#Section 5: Bioprocess Engineering and Process Biotechnology

A baffled fermenter with tank diameter and liquid height equal to 1.2 m is stirred using a six-blade downward-pumping impeller. The impeller diameter is 0.42 m and the stirrer speed is 1.5 s. The viscosity of the fermentation broth is 10^-2 Pa s and the density is 1000 kg m-³. Estimate the mixing time under nonaerated conditions.

#Question id: 28150

#Section 5: Bioprocess Engineering and Process Biotechnology

A purified hydrolytic enzyme (Mr 150 000) is available with the description: Gives a Vmax of 3 200 units/mg protein under optimal conditions. How many micromoles of substrate can be hydrolysed by 1 mg of protein in 1 minute  

#Question id: 31198

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology