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TLS Online TPP Program

#Question id: 10551

plants possess a second type of immunity mediated by a set of highly specific intracellular receptors known as nucleotide binding site–leucine rich repeat (NBS–LRR) receptors. How NBS–LRRs become activated,

#Section 6: Plant, Animal and Microbial Biotechnology
  1. by binding with plant proteins such as guardees or decoys
  2. by binding directly to a pathogen effector with guardees or decoys
  3. by binding as long as it is bound to its guardees
  4. by binding effector molecules with LRR domain
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TLS Online TPP Program

#Question id: 12904

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Match the following:
1. Southern Blotting                               i. Melting temperature of DNA strand
2. DNA microchips                                   ii.oligonucleotide matching to DNA sample
3. ASO-PCR                                                 iii.DNA hybridization to oligo arrays
4. DGGE                                                        iv. labeled probe hybridization to DNA  
TLS Online TPP Program

#Question id: 12905

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Some gene/protein is used to identify the Disorder in humans
Match them correctly:
1. Tyrosine Kinase(TK)                        i. Atherosclerosis
2. HIV transactivator                          ii. Hypertension
3. Angiotensinogen                            iii. Kaposis Sarcoma
4. CET protein                                      iv. Cardiac Hypertrophy
TLS Online TPP Program

#Question id: 12906

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

An alternative method labeling strategy used in karyotyping and gene localisation is _______
TLS Online TPP Program

#Question id: 12907

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

The binding of a regulatory protein or transcription factor to a specific DNA site results in a complex which may be analyzed by the technique termed as _____
TLS Online TPP Program

#Question id: 12908

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

Which statement is incorrect about CRISPR?
TLS Online TPP Program

#Question id: 12909

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

According to the diagram, arrange the steps in correct order: 


1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 
2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 
3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).
 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.