Intercalating agents are flat molecules containing several polycyclic rings that bind to the equally flat purine or pyrimidine bases of DNA, just as the bases bind or stack with each other in the double helix

TLS Online TPP Program

#Id: 429

#Unit 2. Cellular Organization

Mononucleosomes typically have 200 bp DNA. End-trimming reduces the length of DNA first to 165 bp and then generates core particles with 146 bp.

TLS Online TPP Program

#Id: 430

#Unit 2. Cellular Organization

Mnase digestion result on centrifugation and electrophoresis

TLS Online TPP Program

#Id: 431

#Unit 2. Cellular Organization

Core DNA has an invariant length of 146 bp the minimum length of DNA needed to form a stable monomeric nucleosome, and is relatively resistant to digestion by nucleases.

TLS Online TPP Program

#Id: 432

#Unit 2. Cellular Organization

Core DNA invariant length of 146 bp contains core and linker histones so Mnase cuts after H1 binding site. 

TLS Online TPP Program

#Id: 433

#Unit 2. Cellular Organization

The nucleosome is a disc with DNA organized into 12/3 turns around the surface.

TLS Online TPP Program

#Id: 434

#Unit 2. Cellular Organization

The nucleosome is a disc of 11 nm diameter and 6 nm height. 2πrh is surface are and πr2h is volume of nucleosome