What are the principles in flow cytometry?

• Light scattered by a laser or arc lamp

• Specific fluorescence detection

• Hydrodynamically focused stream of particles

• Electrostatic particle separation for sorting

• Multivariate data analysis capability

TLS Online TPP Program

#Id: 9224

#Unit 6. System Physiology – Plant

Warmer environments have the effect of: Lowering the solubility of CO2 to a greater extent than O2

TLS Online TPP Program

#Id: 9225

#Unit 6. System Physiology – Plant

Warmer environments have the effect of: Reducing the stomatal aperture to conserve water. The stomatal closure lowers the uptake of atmospheric CO2, thereby decreasing CO2 at the active site of rubisco.

TLS Online TPP Program

#Id: 9226

#Unit 6. System Physiology – Plant

Carbon cycle, nitrogen cycle, and oxygen cycle atoms circulate through photorespiration

TLS Online TPP Program

#Id: 9227

#Unit 6. System Physiology – Plant

The specificity factor (W) estimates the preference of rubisco for CO2 versus O2

where Vc and Vo  are the maximum velocities for carboxylation and oxygenation, respectively, and Kc and Ko are the Michaelis–Menten constants for CO2 and O2, respectively

TLS Online TPP Program

#Id: 9228

#Unit 6. System Physiology – Plant

8 μM CO2 (0.038%) and 250 μM O2 (21%) at 25°C. Therefore, a specificity factor of 100 gives a Vc/Vo ratio of 3.2.

TLS Online TPP Program

#Id: 9229

#Unit 6. System Physiology – Plant

C2 oxidative photosynthetic carbon cycle is essential for land plants for retrieving the carbon atoms of 2-phosphoglycolate.