In budding yeast CKI p27 = Sic1 in metazoans

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#Unit 1. Molecules and their Interaction Relevant to Biology

Predictor of Natural Disordered Regions or PONDR is one of the best available algorithms for predicting the likelihood that a given amino acid residue is in a region of intrinsic disorder, based on the surrounding amino acid sequence and amino acid composition. 

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#Unit 1. Molecules and their Interaction Relevant to Biology

A PONDR score of 1.0 indicates a probability of 100% that a protein will be disordered.

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#Unit 1. Molecules and their Interaction Relevant to Biology

The mammalian protein p53 also have both structured and unstructured segments. With its unstructured region at the carboxyl terminus four different proteins.

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#Unit 1. Molecules and their Interaction Relevant to Biology

Thermodynamic measurements indicate that native proteins are only marginally stable under physiological conditions. The free energy required to denature them is ∼0.4 kJ ∙ mol−1 per amino acid residue.

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#Unit 1. Molecules and their Interaction Relevant to Biology

Protein stability is the net balance of forces, which determine whether a protein will be in its native folded conformation or a denatured state.

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#Unit 1. Molecules and their Interaction Relevant to Biology

A fully folded 100-residue protein is only about 40 kJ ∙ mol−1 more stable than its unfolded form (for comparison, the energy required to break a typical hydrogen bond is ∼20 kJ ∙ mol−1).