Inflammation

Tissue response to infection or damage which serves to eliminate

Classic signs of acute inflammation are heat (calore), pain (dolore), redness (rubor), swelling (tumor), and loss of function (functio laesa).

TLS Online TPP Program

#Id: 4607

#Unit 13. Methods in Biology

(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles

TLS Online TPP Program

#Id: 4608

#Unit 13. Methods in Biology

CFSE labeling amount of fluorescence emitted is cut in half each time the cell divides.

TLS Online TPP Program

#Id: 4609

#Unit 13. Methods in Biology

The 51Cr Release Assay is used to measure Cell death not apoptosis

TLS Online TPP Program

#Id: 4610

#Unit 13. Methods in Biology

Normal cells treated with sodium chromate allowed to absorb and induced to death signals as T-CTLs Death of the target cells is indicated by the release of 51Cr into the supernatant

TLS Online TPP Program

#Id: 4611

#Unit 13. Methods in Biology

FITC binds to Annexin V to phosphatidyl serine which are found on apoptotic cells, analyzed by FACS

TLS Online TPP Program

#Id: 4612

#Unit 13. Methods in Biology

The TUNEL Assay measures Apoptotically Generated DNA Fragmentation.