This pedigree shows the inheritance of a recessive trait in humans. Individuals that have the trait are homozygous for a recessive allele a. If H and I, who happen to be first cousins, marry and have a child, what is the chance that this child will have the recessive trait?

TLS Online TPP Program

#Question id: 18706

#Unit 13. Methods in Biology

Sources of thermostable DNA polymerases with proofreading (3′–5′ exonuclease) activity are given match them correctly

DNA polymerase                          Source

a. Tma                                      i. Thermotoga maritima

b. Deep vent tm                      ii. Pyrococcus furiosus

c. Tli                                      iii. Thermococcus litoralis

d. Pfu                                      iv.  Pyrococcus sp

e. Pwo                                      v. Pyrococcus woesi

TLS Online TPP Program

#Question id: 18707

#Unit 13. Methods in Biology

primer dimers are formed when 

TLS Online TPP Program

#Question id: 18708

#Unit 13. Methods in Biology

PCR can be analysed amplification, Anneleaning, and extension and analysis is called

TLS Online TPP Program

#Question id: 18709

#Unit 13. Methods in Biology

qPCR can be done by 

i) cyanin dye or SYBR green – to measure amplification

ii) Taqman assay – measure extension

iii) molecular beacons - denaturation

TLS Online TPP Program

#Question id: 18710

#Unit 13. Methods in Biology

qPCR  graph is drawn between ∆Rn and PCR cycle In this graph CT represents

 

TLS Online TPP Program

#Question id: 18711

#Unit 13. Methods in Biology

SYBR green qPCR based on