Nurturing Life Sciences
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#Unit 13. Methods in Biology
Electrophoresis of normal RNA can be done in a 2% agarose gel in about 1 h while, Ribosomal RNAs (18 S and 28 S) are clearly resolved in 2.5–5% acrylamide gradient gel with an overnight run.
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Capillary zone electrophoresis is the example of free solution electrophoresis with highest resolution using high voltage and using Electrophoretic mobility (EFM) & Electroendosmosis force (EEOF).
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Capillary zone electrophoresis can analyze hybridization probes, gene-cloning, DNA sequencing, polymerase chain reaction (PCR), HIV load, site-directed mutagenesis, & antisense therapeutics.
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Point mutations in DNA, quantification of DNA, Chiral compounds all ca be analyzed by using Capillary zone electrophoresis.
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Output of capillary zone electrophoresis known as electropherogram.
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Microchip electrophoresis is also based on free flow.