To more accurately validate the predicted target genes of miRNAs and exactly determine the cleavage sites within the targets, an integrated strategy comprising RLM-RACE, Poly(A) Polymerase-Mediated (PPM)-RACE, and qRT-PCR was developed.

TLS Online TPP Program

#Id: 4755

#Unit 5. Developmental Biology

The SKN-1 protein is a transcription factor that initiates the activation of those genes responsible for forming the pharynx and intestine.

TLS Online TPP Program

#Id: 4756

#Unit 5. Developmental Biology

The SKN-1 protein is a maternally expressed transcription factor that controls the fate of the P1 blastomere(P1🡪EMS 🡪MS), that generates the posterior pharynx.

TLS Online TPP Program

#Id: 4757

#Unit 5. Developmental Biology

Embryos from homozygous skn-1-deficient mothers lack both pharyngeal mesoderm & endoderm derivatives of EMS.

TLS Online TPP Program

#Id: 4758

#Unit 5. Developmental Biology

skn-1-deficient embryos make extra hypodermal (skin) & body wall tissue.

TLS Online TPP Program

#Id: 4759

#Unit 5. Developmental Biology

PAL-1 activity is needed for the normal development of the somatic (but not the germline) descendants of the P2 blastomere, specifies muscle production.

TLS Online TPP Program

#Id: 4760

#Unit 5. Developmental Biology

Embryos lacking PAL-1 have no somatic cell types derived from the C and D stem cells.