To more accurately validate the predicted target genes of miRNAs and exactly determine the cleavage sites within the targets, an integrated strategy comprising RLM-RACE, Poly(A) Polymerase-Mediated (PPM)-RACE, and qRT-PCR was developed.

TLS Online TPP Program

#Id: 960

#Unit 5. Developmental Biology

CGSP, an enzyme that cleaves the proteins linking the vitelline envelope to the cell membrane & also clips off the bindin receptors and any sperm attached to them.

TLS Online TPP Program

#Id: 961

#Unit 5. Developmental Biology

Cortical granule protein hyalin, forms a coating around the egg.

TLS Online TPP Program

#Id: 7571

#Unit 5. Developmental Biology

Mammalian fertilization occurs inside the oviducts  of the female

TLS Online TPP Program

#Id: 7572

#Unit 5. Developmental Biology

Translocation of sperm from the vagina to the oviduct involves several processes that work at different times and places:

Sperm motility

Uterine muscle contractions

Sperm rheotaxis

TLS Online TPP Program

#Id: 7573

#Unit 5. Developmental Biology

Sperm rheotaxis 

migrate against the direction of the flow—using

CatSper calcium channels (like sea urchin sperm) to sense calcium influx and monitor the direction of the

current

TLS Online TPP Program

#Id: 7574

#Unit 5. Developmental Biology

The cells of the oviduct induce the sperm to mature, and mammalian sperm complete their development in the female oviducts. This maturation is called capacitation (the gaining of capacity).