When pairs of human 23 and mouse 20 homologs are compared, the genes located on either side also tend to be homologs. This relationship is called synteny

TLS Online TPP Program

#Id: 6454

#Unit 3. Fundamental Processes

The α-amanitin binding site is beneath a “bridge helix” extending across the cleft between the two largest pol II subunits, Rpb1 and Rpb2

a DNA template, whereas in eukaryotes, transcription takes place on a chromatin template.

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#Id: 6455

#Unit 3. Fundamental Processes

Eukaryotic nuclei contain RNA polymerase I, RNA polymerase II, and RNA polymerase III can be separated by using DEAE-Sephadex ion-exchange chromatography

TLS Online TPP Program

#Id: 6456

#Unit 3. Fundamental Processes

RNA Polymerase can also separated by using epitope tagging. Normally RPB3 is used to create epitope tag.

TLS Online TPP Program

#Id: 6457

#Unit 3. Fundamental Processes

Molecular genetic techniques are used to make a cDNA encoding a recombinant protein to which is fused a short sequence of amino acids called an epitope tagging.

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#Id: 6458

#Unit 3. Fundamental Processes

Two commonly used epitope tags are FLAG, which encodes the amino acid sequence DYKDDDDK (single-letter amino acid code), and myc, which encodes the sequence EQKLISEEDL.

TLS Online TPP Program

#Id: 6459

#Unit 3. Fundamental Processes