Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

#Question id: 4965

#Unit 9. Evolution & Diversity of Life Forms

What is thought to be the correct sequence of these events, from earliest to most recent, in the evolution of life on Earth?

1.  origin of mitochondria

2.  origin of multicellular eukaryotes

3.  origin of chloroplasts

4.  origin of cyanobacteria

5.  origin of fungal-plant symbioses

#Question id: 4966

#Unit 9. Evolution & Diversity of Life Forms

If it were possible to conduct sophisticated microscopic and chemical analyses of microfossils found in 3.2-billion-year-old stromatolites, then within such microfossils, one should be surprised to observe evidence of:

I. double-stranded DNA

II. a nuclear envelope

III. a nucleoid

IV. a nucleolus

V. nucleic acids

#Question id: 4967

#Unit 9. Evolution & Diversity of Life Forms

Recent evidence indicates that the first major diversification of multicellular eukaryotes may have coincided in time with the

#Question id: 4968

#Unit 9. Evolution & Diversity of Life Forms

Which of these observations fails to support the endosymbiotic theory for the origin of eukaryotic cells?

#Question id: 4969

#Unit 9. Evolution & Diversity of Life Forms

The snowball Earth hypothesis provides a possible explanation for the

#Question id: 4970

#Unit 9. Evolution & Diversity of Life Forms

If two continental land masses converge and are united, then the collision should cause