Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

 Which of the following is true for traditional and real time PCR?

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#Question id: 27210

#Unit 1. Molecules and their Interaction Relevant to Biology

Charge on one mol of electron is approximately equal to

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#Question id: 27211

#Unit 1. Molecules and their Interaction Relevant to Biology

 A biological redox reaction always involves

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#Question id: 27226

#Unit 1. Molecules and their Interaction Relevant to Biology

The Protein Components of the Mitochondrial Electron-Transfer Chain match them correctly with their prosthetic groups 

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#Question id: 27357

#Unit 6. System Physiology – Plant

Chlorophyll can exist in three state in many state which state have no unpaired electron are found

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#Question id: 27358

#Unit 6. System Physiology – Plant

Choose the correct statement regarding singlet and triplet state

1. singlet state is short lived, net angular momentum and spin quantum number, s is zero

2. singlet state is long lived, net angular momentum and spin quantum number, s is one

3. Triplet state is long lived , net angular momentum and spin quantum number, s is one

4. Triplet state is short lived net angular momentum and spin quantum number, s is zero

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#Question id: 27374

#Unit 13. Methods in Biology

An aqueous solution contains two compounds X and Y. This solution gave absorbance values of 1.0 and 0.4 at 220 and 280 nm, respectively, in a 1 cm path length cell. Molar absorption coefficients (ε) of the compounds X and Y are as shown in the table below.

The concentration of Y in the solution is___________ mM.