A stirred-tank reactor is to be scaled down from 10 m3 to 0.1 m3. The dimensions of the large tank are: Dt = 2 m; Di = 0.5 m; N = 100 rpm. What would be the required rotational speed of the impeller in the small tank if impeller Re number is constant?

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TLS Online TPP Program

#Question id: 14360

#Section 5: Bioprocess Engineering and Process Biotechnology

1 pg of an enzyme (Mr 160 000) converts 0.192 pmol/min. This enzyme is made up of four subunits. Calculate turnover number 

TLS Online TPP Program

#Question id: 14361

#Section 5: Bioprocess Engineering and Process Biotechnology

1 m of an enzyme (Mr 160 000) converts 0.192 i.imol/min. This enzyme is made up of four  subunits. Calculate catalytic centre activity

TLS Online TPP Program

#Question id: 14362

#Section 5: Bioprocess Engineering and Process Biotechnology

An experiment requires the use of 5 x 107 units of enzyme. The price for the enzyme is quoted for katal amounts. Allowing for a 20% excess for wastage, pipetting, and so forth, what approximate round number of katals of enzyme would you purchase? 

TLS Online TPP Program

#Question id: 14363

#Section 5: Bioprocess Engineering and Process Biotechnology

Enzyme activity was monitored over the last two stages of an enzyme extraction-separation process. 0.4 g protein, from Stage 5, converted 46 mmol substrate in 10 sec, and 0.02 g protein, from Stage 6, converted 49 mmol in 30 sec. Calculate the specific activities in units/g at each of the two steps 

TLS Online TPP Program

#Question id: 14364

#Section 5: Bioprocess Engineering and Process Biotechnology

Enzyme activity was monitored over the last two stages of an enzyme extraction-separation process. 0.4 g protein, from Stage 5, converted 46 mmol substrate in 10 sec, and 0.02 g protein, from Stage 6, converted 49 mmol in 30 sec. Calculate % increase in activity obtained over the two step  

TLS Online TPP Program

#Question id: 14365

#Section 5: Bioprocess Engineering and Process Biotechnology

0.1 mg of a quaternary structured enzyme (Mr 486 000) with four active sites converts 48 iimol substrate/10 sec. Calculate turnover number (min-1)